Optogenetic control of contractile tissue forces during Drosophila morphogenesis
El Departamento de Ingeniería Biomédica de la Universidad de los Andes te invita a participar en la sesión extraordinaria del Seminario IBIO. La Dra. Marisol Herrera Pérez, investigadora postdoctoral de Colombia University presentará la charla: Optogenetic control of contractile tissue forces during Drosophila morphogenesis.
Epithelial tissues undergo dramatic changes in shape during development. These changes are driven in large part by contractile forces generated by the actomyosin cytoskeleton of cells. In addition to physically shaping cells and tissues, mechanical forces can act as cues to influence cell behavior and could help coordinate cell behaviors across multicellular tissues. A major obstacle to dissect the mechanisms of how mechanical forces shape tissues has been the lack of tools for precise manipulation of forces in vivo. To address this, we developed a collection of optogenetic tools to locally and systematically modulate actomyosin contractility in the Drosophila embryo. With these tools, we have demonstrated local, light-gated myosin recruitment and changes in myosin localization patterns across tissues. Using these tools in combination with live confocal imaging and quantitative images analysis, we find that inducing contractile forces in small groups of cells in an epithelial tissue can deform and influence cell behaviors across the tissue in the developing embryo. These studies are shedding light on the roles of mechanical cues in coordinating cell behaviors in multicellular tissues during development.
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